A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction
نویسندگان
چکیده
منابع مشابه
A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction
The CRISPR (clustered regularly interspaced short palindromic repeats) system protects archaea and bacteria by eliminating nucleic acid invaders in a crRNA-guided manner. The Sulfolobus islandicus type III-B Cmr-α system targets invading nucleic acid at both RNA and DNA levels and DNA targeting relies on the directional transcription of the protospacer in vivo. To gain further insight into the ...
متن کاملTarget RNA capture and cleavage by the Cmr type III-B CRISPR-Cas effector complex.
The effector complex of the Cmr/type III-B CRISPR (clustered regularly interspaced short palindromic repeat)-Cas (CRISPR-associated) system cleaves RNAs recognized by the CRISPR RNA (crRNA) of the complex and includes six protein subunits of unknown functions. Using reconstituted Pyrococcus furiosus Cmr complexes, we found that each of the six Cmr proteins plays a critical role in either crRNA ...
متن کاملBipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR-Cas system.
CRISPR-Cas systems eliminate nucleic acid invaders in bacteria and archaea. The effector complex of the Type III-B Cmr system cleaves invader RNAs recognized by the CRISPR RNA (crRNA ) of the complex. Here we show that invader RNAs also activate the Cmr complex to cleave DNA. As has been observed for other Type III systems, Cmr eliminates plasmid invaders in Pyrococcus furiosus by a mechanism t...
متن کاملProgrammable type III-A CRISPR-Cas DNA targeting modules
The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves targeted DNA and RNA molecules. In this work, we have constructed modules of Csm systems from 3 bacterial species and heterologously expressed t...
متن کاملCorrection: RNA and DNA Targeting by a Reconstituted Thermus thermophilus Type III-A CRISPR-Cas System
[This corrects the article DOI: 10.1371/journal.pone.0170552.].
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2016
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkw1274